| First Author | Zhang Y | Year | 2019 |
| Journal | Am J Physiol Cell Physiol | Volume | 317 |
| Issue | 6 | Pages | C1115-C1127 |
| PubMed ID | 31509448 | Mgi Jnum | J:282732 |
| Mgi Id | MGI:6379095 | Doi | 10.1152/ajpcell.00208.2019 |
| Citation | Zhang Y, et al. (2019) cGMP-dependent protein kinase II determines beta-catenin accumulation that is essential for uterine decidualization in mice. Am J Physiol Cell Physiol 317(6):C1115-C1127 |
| abstractText | In the early phase of pregnancy, decidualization is an indispensable event after mammal embryo implantation, accompanied by proliferation and differentiation of uterine stromal cells. Type II cGMP-dependent protein kinase (Prkg2) belongs to the family of serine/threonine kinase, which plays multiple roles in cellular signaling pathways to control proliferation and differentiation. However, the regulatory function and molecular mechanism of Prkg2 in decidualization are still unknown. In this study, we show that Prkg2 has a gradually increased expression pattern during peri-implantation and artificial decidualization, and the expression of Prkg2 is induced by estrogen and progesterone in the ovariectomized mouse uteri and primary cultured uterine stromal cells, the process of which is blocked by treating with estrogen receptor (ER) antagonist (ICI-182,780) and progesterone receptor (PR) antagonist (RU-486). Inhibition of Prkg2 activity by HA-100 promotes uterine stromal cell proliferation but compromises decidualization with decreased expression of prolactin family 8, subfamily a, member 2. In addition, the functional regulation of decidualization by Prkg2 is accomplished by its induced phosphorylation of glycogen synthase kinase-3beta (GSK-3beta) at serine-9, which results in accumulation of beta-catenin in the decidual cells. Taken together, our findings demonstrate that estrogen and progesterone upregulate the expression of Prkg2 in uterine stromal cells depending on ER and PR; Prkg2 promotes phosphorylation of GSK-3beta at serine-9 and inactivates it, leading to the accumulation of beta-catenin and promoting the process of decidualization. In addition to revealing the regulatory mechanism of Prkg2 that ensures the success of uterine decidualization, our findings will contribute to the understanding in the maintenance of early pregnancy. |
