| First Author | Kosmach A | Year | 2021 |
| Journal | Cell Rep | Volume | 37 |
| Issue | 3 | Pages | 109846 |
| PubMed ID | 34686324 | Mgi Jnum | J:328736 |
| Mgi Id | MGI:6881824 | Doi | 10.1016/j.celrep.2021.109846 |
| Citation | Kosmach A, et al. (2021) Monitoring mitochondrial calcium and metabolism in the beating MCU-KO heart. Cell Rep 37(3):109846 |
| abstractText | Optical methods for measuring intracellular ions including Ca(2+) revolutionized our understanding of signal transduction. However, these methods are not extensively applied to intact organs due to issues including inner filter effects, motion, and available probes. Mitochondrial Ca(2+) is postulated to regulate cell energetics and death pathways that are best studied in an intact organ. Here, we develop a method to optically measure mitochondrial Ca(2+) and demonstrate its validity for mitochondrial Ca(2+) and metabolism using hearts from wild-type mice and mice with germline knockout of the mitochondria calcium uniporter (MCU-KO). We previously reported that germline MCU-KO hearts do not show an impaired response to adrenergic stimulation. We find that these MCU-KO hearts do not take up Ca(2+), consistent with no alternative Ca(2+) uptake mechanisms in the absence of MCU. This approach can address the role of mitochondrial Ca(2+) to the myriad of functions attributed to alterations in mitochondrial Ca(2+). |
