Primary Identifier | MGI:3040317 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Vsx1 |
Transmission | Germline | Strain of Origin | 129S4/SvJae-Tg(Prm-cre)70Og |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Targeting took place in ES cells carrying a cre transgene Tg(Prm-cre)70Og, which expresses Cre recombinase under control of the male-germline specific protamine promoter. A floxed neo cassette replaced the coding region for the entire homeodomain and CVC domain, leaving fragments of exons 1 and 5 intact. Cre-mediated excision removed the neo cassette upon transmission from male chimeras. |