| Primary Identifier | MGI:1857481 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Pomc |
| Transmission | Germline | Strain of Origin | 129S2/SvPas |
| Is Recombinase | false | Is Wild Type | false |
| description | Additional reference J:65685 identifies the ES cell line origin as 129S2/SvEv. It is more likely that the mice used in this additional reference were derived from the targeted D3 ES cells (129S2/SvPas) first generated in J:78759 and implanted in mice in J:47711. |
| molecularNote | A PGK-neomycin based targeting vector was used to introduce a premature start codon into exon 3, resulting in the truncation of the translated protein. In situ hybridization on homozygous mutant animals demonstrates that the truncation event had no effect on the distribution or level of transcript. Immunoreactivity demonstrated that Homozygous mutant mice had no detectable beta endorphin activity in the hypothalamus or pituitary |
