| First Author | Ozawa T | Year | 2022 |
| Journal | J Immunol | Volume | 208 |
| Issue | 8 | Pages | 1937-1946 |
| PubMed ID | 35379742 | Mgi Jnum | J:324711 |
| Mgi Id | MGI:7281044 | Doi | 10.4049/jimmunol.2101097 |
| Citation | Ozawa T, et al. (2022) Special AT-Rich Sequence-Binding Protein 1 Supports Survival and Maturation of Naive B Cells Stimulated by B Cell Receptors. J Immunol 208(8):1937-1946 |
| abstractText | Epigenetic mechanisms underpin the elaborate activities of essential transcription factors in lymphocyte development. Special AT-rich sequence-binding protein 1 (SATB1) is a chromatin remodeler that orchestrates the spatial and temporal actions of transcription factors. Previous studies have revealed the significance of SATB1 in T cell lineage. However, whether and how SATB1 controls B cell lineage development is yet to be clarified. In this study, we show that SATB1 is an important factor during splenic B cell maturation. By analyzing SATB1/Tomato reporter mice, we determined the dynamic fluctuation of SATB1 expression in the B cell lineage. Although SATB1 expression decreased to minimal levels during B cell differentiation in the bone marrow, it resurged markedly in naive B cells in the spleen. The expression was dramatically downregulated upon Ag-induced activation. Splenic naive B cells were subdivided into two categories, namely SATB1(high) and SATB1(-/low), according to their SATB1 expression levels. SATB1(high) naive B cells were less susceptible to death and greater proliferative than were SATB1(-/low) cells during incubation with an anti-IgM Ab. Additionally, SATB1(high) cells tended to induce the expression of MHC class II, CD86, and CD83. Accordingly, naive B cells from B lineage-specific SATB1 conditional knockout mice were more susceptible to apoptosis than that in the control group upon anti-IgM Ab stimulation in vitro. Furthermore, conditional knockout mice were less capable of producing Ag-specific B cells after immunization. Collectively, our findings suggest that SATB1 expression increases in naive B cells and plays an important role in their survival and maturation. |
