| First Author | Karpińska K | Year | 2020 |
| Journal | Front Cell Dev Biol | Volume | 8 |
| Pages | 154 | PubMed ID | 32258033 |
| Mgi Jnum | J:308883 | Mgi Id | MGI:6741736 |
| Doi | 10.3389/fcell.2020.00154 | Citation | Karpinska K, et al. (2020) Alpha-Catulin, a New Player in a Rho Dependent Apical Constriction That Contributes to the Mouse Neural Tube Closure. Front Cell Dev Biol 8:154 |
| abstractText | Coordination of actomyosin contraction and cell-cell junctions generates forces that can lead to tissue morphogenetic processes like the formation of neural tube (NT), however, its molecular mechanisms responsible for regulating and coupling this contractile network to cadherin adhesion remain to be fully elucidated. Here, using a gene trapping technology, we unveil the new player in this process, alpha-catulin, which shares sequence homology with vinculin and alpha-catenin. Ablation of alpha-catulin in mouse causes defective NT closure due to impairment of apical constriction, concomitant with apical actin and P-Mlc2 accumulation. Using a 3D culture model system, we showed that alpha-catulin localizes to the apical membrane and its removal alters the distribution of active RhoA and polarization. Actin cytoskeleton and P-Mlc2, downstream targets of RhoA, are not properly organized, with limited accumulation at the junctions, indicating a loss of junction stabilization. Our data suggest that alpha-catulin plays an important role during NT closure by acting as a scaffold for RhoA distribution, resulting in proper spatial activation of myosin to influence actin-myosin dynamics and tension at cell-cell adhesion. |
