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Allele : Tg(CAG-GFP/LC3/RFP/LC3*)2Nmz transgene insertion 2, Noboru Mizushima

Primary Identifier  MGI:6508991 Allele Type  Transgenic
Attribute String  Reporter Gene  Tg(CAG-GFP/LC3/RFP/LC3*)2Nmz
Strain of Origin  C57BL/6J Is Recombinase  false
Is Wild Type  false
molecularNote  This transgene expresses GFP-LC3-RFP-LC3DeltaG, a fluorescent probe to evaluate autophagic flux, under the control of a CAG promoter. The fusion protein is cleaved by endogenous ATG4 proteases into equimolar amounts of GFP-LC3 and RFP-LC3DeltaG. GFP-LC3 is degraded by autophagy, while RFP-LC3DeltaG remains in the cytosol, serving as an internal control. Thus, autophagic flux can be estimated by calculating the GFP/RFP signal ratio. In this line, high expression was observed only in the skeletal muscle.
  • mutations:
  • Insertion
  • synonyms:
  • Tg(CAG-GFP/LC3/RFP/LC3*deltaG)2Nmz,
  • Tg(CAG-GFP/LC3/RFP/LC3*deltaG)2Nmz
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1 Feature

Trail: Allele

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

1 Carried By

Trail: Allele

0 Driven By

4 Publication categories

Trail: Allele