| Primary Identifier | MGI:5790983 | Allele Type | Targeted |
| Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Sparc |
| Transmission | Germline | Strain of Origin | 129 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Sequence from the human ortholog (consisting of the 1 kb 3' UTR from haplotype B which has the C allele of SNP rs1054204, the consensus polyA site and a G-T rich region), preceded by a loxP site flanked neomycin resistance gene cassette, was flanked by 8-10 kb long mouse homology arms. This construct was targeted to the mouse gene and used to replace the endogenous 3' UTR in exon 10 with the human 3' UTR. Subsequent cre-mediated recombination excised the neo cassette. |
