| Primary Identifier | MGI:7262996 | Allele Type | Targeted |
| Attribute String | No functional change | Gene | Adgrl2 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac x C57BL/6J |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was designed to introduce a loxP site upstream of the first coding exon (exon 2). A segment carrying an F1 FRT site, FRT site, 1 kb of the mouse engrailed splice acceptor, 5 kb of Adgrl2 cDNA fused to mVenus green fluorescent protein, a polyA signal, FRT site, PGK-neomycin resistance cassette, tandem FRT and F1 FRT sites, and a loxP site were placed in intron 2. Flp-mediated recombination in the germline resulted in the removal of the cDNA/Venus gene fusion and the neomycin selection cassette, leaving loxP sites flanking exon 1. |
