| First Author | Vyas Y | Year | 2020 |
| Journal | Mol Brain | Volume | 13 |
| Issue | 1 | Pages | 110 |
| PubMed ID | 32758248 | Mgi Jnum | J:303870 |
| Mgi Id | MGI:6510039 | Doi | 10.1186/s13041-020-00650-0 |
| Citation | Vyas Y, et al. (2020) Influence of maternal zinc supplementation on the development of autism-associated behavioural and synaptic deficits in offspring Shank3-knockout mice. Mol Brain 13(1):110 |
| abstractText | Autism Spectrum Disorders (ASD) are characterised by deficits in social interactions and repetitive behaviours. Multiple ASD-associated mutations have been identified in the Shank family of proteins that play a critical role in the structure and plasticity of glutamatergic synapses, leading to impaired synapse function and the presentation of ASD-associated behavioural deficits in mice. Shank proteins are highly regulated by zinc, where zinc binds the Shank SAM domain to drive synaptic protein recruitment and synaptic maturation. Here we have examined the influence of maternal dietary zinc supplementation during pregnancy and lactation on the development of ASD-associated behavioural and synaptic changes in the offspring Shank3 knockout (Shank3(-/-)) mice. Behavioural and electrophysiological experiments were performed in juvenile and adult Shank3(-/-) and wildtype littermate control mice born from mothers fed control (30 ppm, ppm) or supplemented (150 ppm) dietary zinc. We observed that the supplemented maternal zinc diet prevented ASD-associated deficits in social interaction and normalised anxiety behaviours in Shank3(-/-) offspring mice. These effects were maintained into adulthood. Repetitive grooming was also prevented in adult Shank3(-/-) offspring mice. At the synaptic level, maternal zinc supplementation altered postsynaptic NMDA receptor-mediated currents and presynaptic function at glutamatergic synapses onto medium spiny neurons in the cortico-striatal pathway of the Shank3(-/-) offspring mice. These data show that increased maternal dietary zinc during pregnancy and lactation can alter the development of ASD-associated changes at the synaptic and the behavioural levels, and that zinc supplementation from the beginning of brain development can prevent ASD-associated deficits in Shank3(-/-) mice long term. |
