Primary Identifier | MGI:6682042 | Allele Type | Endonuclease-mediated |
Attribute String | Conditional ready, Epitope tag, Inserted expressed sequence | Gene | Igs2 |
Strain of Origin | 129S6/SvEvTac | Is Recombinase | false |
Is Wild Type | false |
molecularNote | CRISPR/cas9 endonuclease-mediated genome editing is used to insert the V2-TIR1 construct containing from 5' to 3'; a human EIF1AX (eukaryotic translation initiation factor 1A X-linked) promoter, a lox-STOP-lox cassette (LSL), the Oryza sativa TIR1 sequence with a hemagglutinin (HA) tag followed by an FRT-flanked puromycin. |