| First Author | Riley JP | Year | 2013 |
| Journal | PLoS One | Volume | 8 |
| Issue | 12 | Pages | e83127 |
| PubMed ID | 24376650 | Mgi Jnum | J:225169 |
| Mgi Id | MGI:5691652 | Doi | 10.1371/journal.pone.0083127 |
| Citation | Riley JP, et al. (2013) PARP-14 binds specific DNA sequences to promote Th2 cell gene expression. PLoS One 8(12):e83127 |
| abstractText | PARP-14, a member of the poly ADP-ribose polymerase super family, promotes T helper cell 2 (Th2) differentiation by regulating interleukin-4 (IL-4) and STAT6-dependent transcription. Yet, whether PARP-14 globally impacts gene regulation has not been determined. In this report, using an RNA pol II ChIP-seq approach, we identify genes in Th2 cells that are regulated by PARP-14, and either dependent or independent of ADP-ribosyltransferase catalytic activity. Our data demonstrate that PARP-14 enhances the expression of Th2 genes as it represses the expression of Th1-associated genes. Among the relevant targets are Signal Transducer and Activator of Transcription genes required for polarizing Th1 and Th2 cells. To define a mechanism for PARP-14 function, we use an informatics approach to identify putative PARP-14 DNA binding sites. Two putative PARP-14 binding motifs are identified in multiple Th2 cytokine genes, and we demonstrate that PARP-14 interacts with each motif using in vitro binding assays. Taken together our results indicate that PARP-14 is an important factor for T helper cell differentiation and it binds to specific DNA sequences to mediate its function. |
