| Primary Identifier | MGI:4332076 | Allele Type | Gene trapped |
| Attribute String | Null/knockout, Reporter | Gene | Kmt2d |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Trapped gene association determined by genome coordinate overlap and strand. An expression cassette encoding a beta-galactosidase neomycin resistance fusion protein inserted into intron 50. Inclusion of a splice acceptor sequence and a 3' end cleavage and polyadenylation signal at the 5' and 3' ends of the beta-Geo cassette, respectively, is predicted to generated a truncated protein with peptide sequence corresponding to the first 50 exons fused to beta-Geo but lacking the SET domain and methyltransferase activity. Real-time PCR confirmed a 50% reduction of mRNA containing exon 52 in heterozygotes. |
