| First Author | Adamiak M | Year | 2016 |
| Journal | Stem Cell Rev Rep | Volume | 12 |
| Issue | 6 | Pages | 613-620 |
| PubMed ID | 27704316 | Mgi Jnum | J:318413 |
| Mgi Id | MGI:6859562 | Doi | 10.1007/s12015-016-9689-x |
| Citation | Adamiak M, et al. (2016) The Involvment of Hematopoietic-Specific PLC -beta2 in Homing and Engraftment of Hematopoietic Stem/Progenitor Cells. Stem Cell Rev Rep 12(6):613-620 |
| abstractText | Migration and bone marrow (BM) homing of hematopoietic stem progenitor cells (HSPCs) is regulated by several signaling pathways, and here we provide evidence for the involvement in this process of hematopoietic-specific phospholipase C-beta2 (PLC-beta2). This enzyme is involved in release of intracellular calcium and activation of protein kinase C (PKC). Recently we reported that PLC-beta2 promotes mobilization of HSPCs from BM into peripheral blood (PB), and this effect is mediated by the involvement of PLC-beta2 in the release of proteolytic enzymes from granulocytes and its role in disintegration of membrane lipid rafts. Here we report that, besides the role of PLC-beta2 in the release of HSPCs from BM niches, PLC-beta2 regulates the migration of HSPCs in response to chemotactic gradients of BM homing factors, including SDF-1, S1P, C1P, and ATP. Specifically, HSPCs from PLC-beta2-KO mice show impaired homing and engraftment in vivo after transplantation into lethally irradiated mice. This decrease in migration of HSPCs can be explained by impaired calcium release in PLC-beta2-KO mice and a high baseline level of heme oxygenase 1 (HO-1), an enzyme that negatively regulates cell migration. |
