| Primary Identifier | MGI:4345347 | Allele Type | Gene trapped |
| Attribute String | Null/knockout, Reporter | Gene | Sec23b |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | Sanger Inst. Gene Trap Res. |
| molecularNote | The gene trap vector inserted into intron 19. RT-PCR analysis indicated a more than 400-fold reduction in expression of wild-type transcript in homozygous mice. Immunoblot analysis failed to detect wild-type protein expression in MEFs from homozygous mice. The fusion protein consisting of the first 19 exons fused to the beta-geo reporter is detected in heterozygous and homozygous cells. The fusion protein is coimmunoprecipitated with SEC24A in MEF lysates. |
