| Primary Identifier | MGI:4353154 | Allele Type | Targeted |
| Attribute String | Inducible, Null/knockout, Recombinase | Gene | Pax7 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | Beginning with the Pax7tm1.2Sjc allele in which exon 2 was knocked out leaving a single loxP site, part of exon 1 was replaced with a cre/ERT2 (ESR1) fusion followed by an IRES-DsRed and an frt flanked neo cassette. Germ line, flp mediated recombination removed the neo cassette. The absence of full-length Pax7 protein expression was confirmed by western blot analysis on P10 tibialis anterior muscle extracts. Expression of the fusion cre/ERT2 protein was confirmed by a cross with a reporter line. DsRed fluorescence was not detectable by epifluorescence nor was DsRed protein by immunostaining. |
