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Allele : Pax7<tm2.1(cre/ERT2)Fan> paired box 7; targeted mutation 2.1, Chen-Ming Fan

Primary Identifier  MGI:4353154 Allele Type  Targeted
Attribute String  Inducible, Null/knockout, Recombinase Gene  Pax7
Transmission  Germline Strain of Origin  (129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Induced With  tamoxifen Is Recombinase  true
Is Wild Type  false
molecularNote  Beginning with the Pax7tm1.2Sjc allele in which exon 2 was knocked out leaving a single loxP site, part of exon 1 was replaced with a cre/ERT2 (ESR1) fusion followed by an IRES-DsRed and an frt flanked neo cassette. Germ line, flp mediated recombination removed the neo cassette. The absence of full-length Pax7 protein expression was confirmed by western blot analysis on P10 tibialis anterior muscle extracts. Expression of the fusion cre/ERT2 protein was confirmed by a cross with a reporter line. DsRed fluorescence was not detectable by epifluorescence nor was DsRed protein by immunostaining.
  • mutations:
  • Intragenic deletion,
  • Insertion
  • synonyms:
  • Pax7<CE>,
  • Pax7<Cre-ERT2>,
  • Pax7<CE>,
  • Pax7<Cre-ERT2>
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1 Feature

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Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

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1 Driven By

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112 Publication categories

Trail: Allele