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Publication : Generating tamoxifen-inducible Cre alleles to investigate myogenesis in mice.

First Author  Lepper C Year  2012
Journal  Methods Mol Biol Volume  798
Pages  297-308 PubMed ID  22130844
Mgi Jnum  J:231137 Mgi Id  MGI:5766890
Doi  10.1007/978-1-61779-343-1_17 Citation  Lepper C, et al. (2012) Generating tamoxifen-inducible Cre alleles to investigate myogenesis in mice. Methods Mol Biol 798:297-308
abstractText  Gene inactivation has become the gold standard for determining gene function in the mouse. Many genes inactivated in the germ line cause early lethality that precludes phenotypic assessment at a later time point. Conditional gene inactivation using Cre recombinase expressed via a tissue specific promoter/enhancer allows phenotypic analyses of selected tissues, but lacks temporal control. Recent development of the tamoxifen-inducible Cre-ER (T2) offers both cell type-specific and temporal control of conditional gene inactivation. As an example, we describe the design and step-wise construction of a Cre-ER (T2) knock-in allele at the Pax7 locus using the recombineering method - Pax7 is selectively expressed in embryonic muscle progenitors and adult muscle stem cells. The resulting Pax7-Cre- ER (T2) (Pax7 (CE)) allele has been successfully applied to embryos and adults for tamoxifen-regulated myogenic lineage tracing and gene inactivation (Nature 460:627-631, 2009; Genesis 48:424-436, 2010).
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