| First Author | Lepper C | Year | 2012 |
| Journal | Methods Mol Biol | Volume | 798 |
| Pages | 297-308 | PubMed ID | 22130844 |
| Mgi Jnum | J:231137 | Mgi Id | MGI:5766890 |
| Doi | 10.1007/978-1-61779-343-1_17 | Citation | Lepper C, et al. (2012) Generating tamoxifen-inducible Cre alleles to investigate myogenesis in mice. Methods Mol Biol 798:297-308 |
| abstractText | Gene inactivation has become the gold standard for determining gene function in the mouse. Many genes inactivated in the germ line cause early lethality that precludes phenotypic assessment at a later time point. Conditional gene inactivation using Cre recombinase expressed via a tissue specific promoter/enhancer allows phenotypic analyses of selected tissues, but lacks temporal control. Recent development of the tamoxifen-inducible Cre-ER (T2) offers both cell type-specific and temporal control of conditional gene inactivation. As an example, we describe the design and step-wise construction of a Cre-ER (T2) knock-in allele at the Pax7 locus using the recombineering method - Pax7 is selectively expressed in embryonic muscle progenitors and adult muscle stem cells. The resulting Pax7-Cre- ER (T2) (Pax7 (CE)) allele has been successfully applied to embryos and adults for tamoxifen-regulated myogenic lineage tracing and gene inactivation (Nature 460:627-631, 2009; Genesis 48:424-436, 2010). |
