| Primary Identifier | MGI:4352941 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Antxr1 |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted upstream of exon 13, which encodes the transmembrane domain, and an frt flanked neo cassette and second loxP site were inserted downstream of exon 13. Cre mediated recombination then removed exon 13 l and (when present) the neo cassette leaving behind a single loxP site. Western blot analysis confirmed the absence of protein expression in lysates from homozygous MEFs but a mutant protein was detected in conditioned media from homozygous MEFs. |
