| Primary Identifier | MGI:4355015 | Allele Type | Targeted |
| Attribute String | Inducible, Knockdown, RMCE-ready | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | C57BL/6 |
| Induced With | doxycycline/tetracycline | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A short hairpin RNA directed against the insulin receptor (Insr) gene was targeted into the Rosa26 locus. The shRNA sequence is under the regulation of the tetO operator and a codon optimized tet repressor (itetR) under the control of a CAG promoter. The construct was transfected into Art B6-RMCE 68 ES cells derived from the C57BL/6 strain through Recombinase Mediated Cassette Exchange. |
