Primary Identifier | MGI:4359084 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Mnx1 |
Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exon 3 of the targeted gene was flanked by loxP sites and a neomycin cassette flanked by FRT sites was introduced to intron 2. The targeting vector was electroporated into 129S1/Sv Oca2+ Tyr+ Kitl +-derived W9.5 embryonic stem (ES) cells. Chimeric animals were crossed to C57BL/6 and then to CB6F1 background FLP mice to remove the neomycin cassette before the strain was bred to homozygosity. |