| First Author | Naffaa MM | Year | 2024 |
| Journal | Sci Signal | Volume | 17 |
| Issue | 855 | Pages | eadk8810 |
| PubMed ID | 39316665 | Mgi Jnum | J:355616 |
| Mgi Id | MGI:7748901 | Doi | 10.1126/scisignal.adk8810 |
| Citation | Naffaa MM, et al. (2024) A cholinergic signaling pathway underlying cortical circuit activation of quiescent neural stem cells in the lateral ventricle. Sci Signal 17(855):eadk8810 |
| abstractText | Neural stem cells (NSCs) in the subventricular zone (SVZ) located along the lateral ventricles (LVs) of the mammalian brain continue to self-renew to produce new neurons after birth and into adulthood. Quiescent LV cells, which are situated close to the ependymal cells lining the LVs, are activated by choline acetyltransferase-positive (ChAT(+)) neurons within the subependymal (subep) region of the SVZ when these neurons are stimulated by projections from the anterior cingulate cortex (ACC). Here, we uncovered a signaling pathway activated by the ACC-subep-ChAT(+) circuit responsible for the activation and proliferation of quiescent LV NSCs specifically in the ventral area of the SVZ. This circuit activated muscarinic M3 receptors on quiescent LV NSCs, which subsequently induced signaling mediated by the inositol 1,4,5-trisphosphate receptor type 1 (IP3R1). Downstream of IP3R1 activation, which would be expected to increase intracellular Ca(2+), Ca(2+)-/calmodulin-dependent protein kinase II delta and the MAPK10 signaling pathway were stimulated and required for the proliferation of quiescent LV NSCs in the SVZ. These findings reveal the mechanisms that regulate quiescent LV NSCs and underscore the critical role of projections from the ACC in promoting their proliferative activity within the ventral SVZ. |
