| First Author | Dumrongprechachan V | Year | 2022 |
| Journal | Elife | Volume | 11 |
| PubMed ID | 36239373 | Mgi Jnum | J:340416 |
| Mgi Id | MGI:7379902 | Doi | 10.7554/eLife.78847 |
| Citation | Dumrongprechachan V, et al. (2022) Dynamic proteomic and phosphoproteomic atlas of corticostriatal axons in neurodevelopment. Elife 11:e78847 |
| abstractText | Mammalian axonal development begins in embryonic stages and continues postnatally. After birth, axonal proteomic landscape changes rapidly, coordinated by transcription, protein turnover, and post-translational modifications. Comprehensive profiling of axonal proteomes across neurodevelopment is limited, with most studies lacking cell-type and neural circuit specificity, resulting in substantial information loss. We create a Cre-dependent APEX2 reporter mouse line and map cell-type-specific proteome of corticostriatal projections across postnatal development. We synthesize analysis frameworks to define temporal patterns of axonal proteome and phosphoproteome, identifying co-regulated proteins and phosphorylations associated with genetic risk for human brain disorders. We discover proline-directed kinases as major developmental regulators. APEX2 transgenic reporter proximity labeling offers flexible strategies for subcellular proteomics with cell type specificity in early neurodevelopment, a critical period for neuropsychiatric disease. |
