| Primary Identifier | MGI:4412099 | Allele Type | Targeted |
| Gene | Prnp | Transmission | Germline |
| Strain of Origin | 129P2/OlaHsd | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The third exon was replaced with an HPRT mini gene that was then replaced with a representative sequence containing two amino acid substitutions found in the human sequence (L108M and V111M). These substitutions allow for transmission barrier against mouse scrapies and immunoreactivity to the 3F4 antibody. A third mutation (D177N) mimicks the mutation associated with human fatal familial insominia. Protein expression levels in the brains of homozygous mice are reduced compared to controls. |
