| Primary Identifier | MGI:4415188 | Allele Type | Targeted |
| Gene | Idua | Transmission | Germline |
| Strain of Origin | 129/Sv | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Nucleotide substitutions (TGG to TAG) were introduced into exon 9 that result in the amino acid substitution of tryptophan with a stop at position 392 (W392X). A floxed neo cassette inserted between exons 8 and 9 was removed by cre mediated recombination leaving a single loxP site. The mutation mimicks one identified in patients with mucopolysaccharidosis type I-Hurler (MPS I-H), also known as Hurler syndrome. RT-PCR confirmed a 30% to 50% reduction in steady-state transcript levels. |
