| First Author | Tsunoda T | Year | 2018 |
| Journal | Int J Mol Med | Volume | 42 |
| Issue | 5 | Pages | 2595-2603 |
| PubMed ID | 30106088 | Mgi Jnum | J:270991 |
| Mgi Id | MGI:6278360 | Doi | 10.3892/ijmm.2018.3806 |
| Citation | Tsunoda T, et al. (2018) Zfat expression in ZsGreen reporter gene knockin mice: Implications for a novel function of Zfat in definitive erythropoiesis. Int J Mol Med 42(5):2595-2603 |
| abstractText | Zinc finger and AThook domain containing (Zfat) is a transcriptional regulator harboring an AThook domain and 18 repeats of a C2H2 zincfinger motif, which binds directly to the proximal region of transcription start sites in Zfattarget genes. It was previously reported that deletion of the Zfat gene in mice yields embryonic lethality by embryonic day 8.5 and impairs primitive hematopoiesis in yolk sac blood islands. In addition, Zfat has been reported to be involved in thymic Tcell development and peripheral Tcell homeostasis. In the present study, in order to obtain a precise understanding of the expression and function of Zfat, a knockin mouse strain (ZfatZsG/+ mice), which expressed ZsGreen in the Zfat locus, was established. ZsGreen signals in tissues and cells of ZfatZsG/+ mice were examined by flow cytometric and histological analyses. Consistent with our previous studies, ZsGreen signals in ZfatZsG/+ mice were detected in the embryo and yolk sac blood islands, as well as in thymocytes, B and T cells. In the ZfatZsG/+ thymus, ZsGreen+ cells were identified not only in Tcell populations but also in thymic epithelial cells, suggesting the role of Zfat in antigenpresenting cells during thymic Tcell development. ZsGreen signals were observed in definitive erythroid progenitor cells in the fetal liver and adult bone marrow of ZfatZsG/+ mice. The proportion of ZsGreen+ cells in these tissues was highest at the early stage of erythroid differentiation, suggesting that Zfat serves particular roles in definitive erythropoiesis. Histological studies demonstrated that ZsGreen signals were detected in the pyramidal cells in the hippocampal CA1 region and the Purkinje cells in the cerebellum, suggesting novel functions of Zfat in nervous tissues. Taken together, these results indicated that the ZfatZsG/+ reporter mouse may be considered a useful tool for elucidating the expression and function of Zfat. |
