| First Author | Gray JM | Year | 2024 |
| Journal | Brain Behav Immun | Volume | 115 |
| Pages | 617-630 | PubMed ID | 37967662 |
| Mgi Jnum | J:343581 | Mgi Id | MGI:7565063 |
| Doi | 10.1016/j.bbi.2023.11.011 | Citation | Gray JM, et al. (2024) The inflammatory response to birth requires MyD88 and is driven by both mother and offspring. Brain Behav Immun 115:617-630 |
| abstractText | Birth is an inflammatory event for the newborn, characterized by elevations in interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-alpha peripherally and/or centrally, as well as changes in brain microglia. However, the mechanism(s) underlying these responses is unknown. Toll-like receptors (TLRs) play crucial roles in innate immunity and initiate inflammatory cascades upon recognition of endogenous or exogenous antigens. Most TLR signaling depends on the adaptor molecule myeloid differentiation primary response 88 (MyD88). We independently varied MyD88 gene status in mouse dams and their offspring to determine whether the inflammatory response to birth depends on MyD88 signaling and, if so, whether that signaling occurs in the offspring, the mother, or both. We find that the perinatal surges in plasma IL-6 and brain expression of TNF-alpha depend solely on MyD88 gene status of the offspring, whereas postnatal increases in plasma IL-10 and TNF-alpha depend on MyD88 in both the pup and dam. Interestingly, MyD88 genotype of the dam primarily drives differences in offspring brain microglial density and has robust effects on developmental neuronal cell death. Milk cytokines were evaluated as a possible source of postnatal maternal influence; although we found high levels of CXCL1/GROalpha and several other cytokines in ingested post-partum milk, their presence did not require MyD88. Thus, the inflammatory response previously described in the late-term fetus and newborn depends on MyD88 (and, by extension, TLRs), with signaling in both the dam and offspring contributing. Unexpectedly, naturally-occuring neuronal cell death in the newborn is modulated primarily by maternal MyD88 gene status. |
