| First Author | Kannan M | Year | 2022 |
| Journal | Science | Volume | 378 |
| Issue | 6619 | Pages | eabm8797 |
| PubMed ID | 36378956 | Mgi Jnum | J:332357 |
| Mgi Id | MGI:7386939 | Doi | 10.1126/science.abm8797 |
| Citation | Kannan M, et al. (2022) Dual-polarity voltage imaging of the concurrent dynamics of multiple neuron types. Science 378(6619):eabm8797 |
| abstractText | Genetically encoded fluorescent voltage indicators are ideally suited to reveal the millisecond-scale interactions among and between targeted cell populations. However, current indicators lack the requisite sensitivity for in vivo multipopulation imaging. We describe next-generation green and red voltage sensors, Ace-mNeon2 and VARNAM2, and their reverse response-polarity variants pAce and pAceR. Our indicators enable 0.4- to 1-kilohertz voltage recordings from >50 spiking neurons per field of view in awake mice and ~30-minute continuous imaging in flies. Using dual-polarity multiplexed imaging, we uncovered brain state-dependent antagonism between neocortical somatostatin-expressing (SST<sup>+</sup>) and vasoactive intestinal peptide-expressing (VIP<sup>+</sup>) interneurons and contributions to hippocampal field potentials from cell ensembles with distinct axonal projections. By combining three mutually compatible indicators, we performed simultaneous triple-population imaging. These approaches will empower investigations of the dynamic interplay between neuronal subclasses at single-spike resolution. |
