| Primary Identifier | MGI:4432601 | Allele Type | Targeted |
| Attribute String | Conditional ready, Hypomorph, Null/knockout, Reporter | Gene | Rnf130 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| description | Special note about the mutation generated in J:338210: authors state that mice homozygous for the Rnf130tm1a(EUCOMM)Wtsi allele exhibit 30% residual Rnf130 mRNA expression in the liver and brain, indicating a hypomorphic mutation. Furthermore, heterozygotes only show a 30% reduction in mRNA expression (versus expected 50%). None of the commercially available antibodies tested were found to be specific for the endogenous mouse RNF130. |
| molecularNote | The L1L2_Pgk_P cassette was inserted at position 49961273 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 49962754. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml |
