| First Author | Chen C | Year | 2021 |
| Journal | Protein Cell | Volume | 12 |
| Issue | 6 | Pages | 455-474 |
| PubMed ID | 33886094 | Mgi Jnum | J:309490 |
| Mgi Id | MGI:6756250 | Doi | 10.1007/s13238-021-00837-8 |
| Citation | Chen C, et al. (2021) Nuclear m(6)A reader YTHDC1 regulates the scaffold function of LINE1 RNA in mouse ESCs and early embryos. Protein Cell 12(6):455-474 |
| abstractText | N(6)-methyladenosine (m(6)A) on chromosome-associated regulatory RNAs (carRNAs), including repeat RNAs, plays important roles in tuning the chromatin state and transcription, but the intrinsic mechanism remains unclear. Here, we report that YTHDC1 plays indispensable roles in the self-renewal and differentiation potency of mouse embryonic stem cells (ESCs), which highly depends on the m(6)A-binding ability. Ythdc1 is required for sufficient rRNA synthesis and repression of the 2-cell (2C) transcriptional program in ESCs, which recapitulates the transcriptome regulation by the LINE1 scaffold. Detailed analyses revealed that YTHDC1 recognizes m(6)A on LINE1 RNAs in the nucleus and regulates the formation of the LINE1-NCL partnership and the chromatin recruitment of KAP1. Moreover, the establishment of H3K9me3 on 2C-related retrotransposons is interrupted in Ythdc1-depleted ESCs and inner cell mass (ICM) cells, which consequently increases the transcriptional activities. Our study reveals a role of m(6)A in regulating the RNA scaffold, providing a new model for the RNA-chromatin cross-talk. |
