| First Author | Klewer T | Year | 2022 |
| Journal | Eur J Immunol | Volume | 52 |
| Issue | 1 | Pages | 44-53 |
| PubMed ID | 34606636 | Mgi Jnum | J:325457 |
| Mgi Id | MGI:7278877 | Doi | 10.1002/eji.202049087 |
| Citation | Klewer T, et al. (2022) E-Cadherin restricts mast cell degranulation in mice. Eur J Immunol 52(1):44-53 |
| abstractText | Crosslinking of FcepsilonRI-bound IgE triggers the release of a large number of biologically active, potentially anaphylactic compounds by mast cells. FcepsilonRI activation ought to be well-controlled to restrict adverse activation. As mast cells are embedded in tissues, adhesion molecules may contribute to limiting premature activation. Here, we report that E-Cadherin serves that purpose. Having confirmed that cultured mast cells express E-Cadherin, a mast-cell-specific E-Cadherin deficiency, Mcpt5-Cre E-Cdh(fl/fl) mice, was used to analyze mast cell degranulation in vitro and in vivo. Cultured peritoneal mast cells from Mcpt5-Cre E-Cdh(fl/fl) mice were normal with respect to many parameters but showed much-enhanced degranulation in three independent assays. Soluble E-Cadherin reduced the degranulation of control cells. The release of some newly synthesized inflammatory cytokines was decreased by E-Cadherin deficiency. Compared to controls, Mcpt5-Cre E-Cdh(fl/fl) mice reacted much stronger to IgE-dependent stimuli, developing anaphylactic shock. We suggest E-Cadherin-mediated tissue interactions restrict mast cell degranulation to prevent their precocious activation. |
