| Primary Identifier | MGI:4436864 | Allele Type | Targeted |
| Attribute String | Not Specified | Gene | H2-Ab1 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP was inserted upstream of exon 3 and a STOP cassette followed by a floxed neo cassette and modified genomic sequence for exon 3 through 6. The modified exon 4 contains a nucleotide substitution that results in the amino acid substitution of arginine for lysine at position 225 (K225R). This amino acid is responsible for the ubiquitination-mediated degradation of the peptide. Cre mediated recombination removes the wild-type exon 3 through 6 and the neo cassette allowing for expression of a transcript containing the nucleotide substitution. |
