Primary Identifier | MGI:4440474 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Ret |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting vector composed of a 1.2 kb fragment of murine Nkx6-2 containing Ret exons 12 and 13 flanked by loxP sites upstream of an frt-flanked neomycin cassette was generated from a BAC clone containing the Ret gene. The vector was incorporated into E14 ES cells for homologous recombination. Two indepent recombined clones were identified and germline transmission was verified by PCR. |