| Primary Identifier | MGI:4453303 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cldn16 |
| Transmission | Germline | Strain of Origin | 129/Sv |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An FRT/loxP-flanked pgk-neo cassette and an additional loxP sequence were inserted to flank exon 2, which allowed subsequent deletion of the exon as well as the neomycin resistance cassette. Elimination of exon 2 leads to a frameshift and early stop codon and truncation of the protein. The deletion of exon 2 as well as the neomycin resistance gene was accomplished via Cre-mediated recombination. Western blot analysis showed the absence of protein expression in kidney tissue from homozygous mice. |
