| Primary Identifier | MGI:4819008 | Allele Type | Targeted |
| Attribute String | Hypomorph | Gene | Pms2 |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An E702K mutation was introduced into exon 12 disrupting the DQHA(X)2E(X)4E metal binding motif. In addition, a floxed hygro/neo selection cassette was introduced in intron 12. Cre mediated recombination then removed the selection cassette. RT-PCR and qPCR indicted that expression levels of the mutant allele were similar to wild-type in MEFs and primary B cells. Western blot analysis indicated about a 25% reduction in protein levels in the spleens, brains, and testes of homozygous mice compared to wild-type tissues. |
