Primary Identifier | MGI:4829435 | Allele Type | Transgenic |
Attribute String | Reporter | Gene | Tg(Pou5f1-Cerulean,-mCherry)1Bhr |
Strain of Origin | (C57BL/6 x SJL)F2 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | The Pou5f1 promoter was used to drive expression of Cerulean fused to histone H2B and mCherry with a 5' fusion to the acrosin secretion signal and a 3' fusion to glyosylphosphatidylinositol. The entire construct is flanked by cHS4 transcriptional insulator sequences. Cerulean expression is restricted to the nucleus and mCherry expression is found on the plasma membrane. |