| Primary Identifier | MGI:4838097 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Alb1-GP33/H2-K)1Hpi |
| Strain of Origin | (C57BL/6 x DBA/2)F2 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The mouse albumin promoter fused to the promoterless genomic H-2Kb gene, in which the signal sequence of the Kb molecule encoded by exon 1 was replaced by the leader sequence (aa 1-60) of the LCMV glycoprotein (GP) encoding the GP33 epitope (aa 33-41). The construct does not encode a functional Kb molecule, since insertion of the LCMV GP fragment leads to a frame shift and a stop codon in the second exon. RT-PCR analysis revealed GP33 transgene expression in the thymus and liver (100-1000x the level of the thymus). |
