| First Author | Christensen AJ | Year | 2016 |
| Journal | Cell Rep | Volume | 17 |
| Issue | 6 | Pages | 1699-1710 |
| PubMed ID | 27806306 | Mgi Jnum | J:242335 |
| Mgi Id | MGI:5904927 | Doi | 10.1016/j.celrep.2016.10.010 |
| Citation | Christensen AJ, et al. (2016) In Vivo Interrogation of Spinal Mechanosensory Circuits. Cell Rep 17(6):1699-1710 |
| abstractText | Spinal dorsal horn circuits receive, process, and transmit somatosensory information. To understand how specific components of these circuits contribute to behavior, it is critical to be able to directly modulate their activity in unanesthetized in vivo conditions. Here, we develop experimental tools that enable optogenetic control of spinal circuitry in freely moving mice using commonly available materials. We use these tools to examine mechanosensory processing in the spinal cord and observe that optogenetic activation of somatostatin-positive interneurons facilitates both mechanosensory and itch-related behavior, while reversible chemogenetic inhibition of these neurons suppresses mechanosensation. These results extend recent findings regarding the processing of mechanosensory information in the spinal cord and indicate the potential for activity-induced release of the somatostatin neuropeptide to affect processing of itch. The spinal implant approach we describe here is likely to enable a wide range of studies to elucidate spinal circuits underlying pain, touch, itch, and movement. |
