| First Author | Reddy JW | Year | 2019 |
| Journal | Front Neurosci | Volume | 13 |
| Pages | 745 | PubMed ID | 31456654 |
| Mgi Jnum | J:359411 | Mgi Id | MGI:6782076 |
| Doi | 10.3389/fnins.2019.00745 | Citation | Reddy JW, et al. (2019) High Density, Double-Sided, Flexible Optoelectronic Neural Probes With Embedded muLEDs. Front Neurosci 13:745 |
| abstractText | Optical stimulation and imaging of neurons deep in the brain require implantable optical neural probes. External optical access to deeper regions of the brain is limited by scattering and absorption of light as it propagates through tissue. Implantable optoelectronic probes capable of high-resolution light delivery and high-density neural recording are needed for closed-loop manipulation of neural circuits. Micro-light-emitting diodes (muLEDs) have been used for optical stimulation, but predominantly on rigid silicon or sapphire substrates. Flexible polymer neural probes would be preferable for chronic applications since they cause less damage to brain tissue. Flexible muLED neural probes have been recently implemented by flip-chip bonding of commercially available muLED chips onto flexible substrates. Here, we demonstrate a monolithic design for flexible optoelectronic neural interfaces with embedded gallium nitride muLEDs that can be microfabricated at wafer-scale. Parylene C is used as the substrate and insulator due to its biocompatibility, compliance, and optical transparency. We demonstrate one-dimensional and two-dimensional individually-addressable muLED arrays. Our muLEDs have sizes as small as 22 x 22 mum in arrays of up to 32 muLEDs per probe shank. These devices emit blue light at a wavelength of 445 nm, suitable for stimulation of channelrhodopsin-2, with output powers greater than 200 muW at 2 mA. Our flexible optoelectronic probes are double-sided and can illuminate brain tissue from both sides. Recording electrodes are co-fabricated with muLEDs on the front- and backside of the optoelectronic probes for electrophysiology recording of neuronal activity from the volumes of tissue on the front- and backside simultaneously with bi-directional optical stimulation. |
