| Primary Identifier | MGI:4839792 | Allele Type | Targeted |
| Attribute String | Conditional ready, Inserted expressed sequence, Reporter | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | mixed |
| Is Recombinase | false | Is Wild Type | false |
| description | YFP3-1 ES cell line = YFP3-1. These cells were derived from embryos resulting from an intercross between Gt(ROSA)26Sortm1Sor; Tg(CAG-cre/Esr1*)5Amc male mice and female Gt(ROSA)26Sortm1(EYFP)Cos mice. This male ES cell line carries Tg(CAG-cre/Esr1*)5Amc and is heterozygous Gt(ROSA)26Sortm1Sor/tm1(EYFP)Cos on a background involving 129S4/SvJae, 129X1/SvJ, C57BL/6, CBA, and Swiss Webster. |
| molecularNote | A tetracycline regulatory unit and polyadenylation signals were inserted upstream of a tetO and minimal CMV promoter. This was followed by a floxed PGK-neomycin-polyA cassette and then by a cDNA for the human smoothened homolog containing a Trp to Leu mutation in codon 535 and fused to YFP. Expression of the SMO* knock-in occurs only after cre excision of the PGK-neo cassette which can be accomplished by tamoxifen treatment to activate cre expression from the TG(CAG-cre/Esr1*)5Amc transgene carried by the YFP3-1 ES cell line used to create this mutation. |
