| First Author | Calvo E | Year | 2020 |
| Journal | Sci Adv | Volume | 6 |
| Issue | 26 | Pages | eaba7509 |
| PubMed ID | 32637615 | Mgi Jnum | J:339735 |
| Mgi Id | MGI:7512860 | Doi | 10.1126/sciadv.aba7509 |
| Citation | Calvo E, et al. (2020) Functional role of respiratory supercomplexes in mice: SCAF1 relevance and segmentation of the Q(pool). Sci Adv 6(26):eaba7509 |
| abstractText | Mitochondrial respiratory complexes assemble into supercomplexes (SC). Q-respirasome (III(2) + IV) requires the supercomplex assembly factor (SCAF1) protein. The role of this factor in the N-respirasome (I + III(2) + IV) and the physiological role of SCs are controversial. Here, we study C57BL/6J mice harboring nonfunctional SCAF1, the full knockout for SCAF1, or the wild-type version of the protein and found that exercise performance is SCAF1 dependent. By combining quantitative data-independent proteomics, 2D Blue native gel electrophoresis, and functional analysis of enriched respirasome fractions, we show that SCAF1 confers structural attachment between III(2) and IV within the N-respirasome, increases NADH-dependent respiration, and reduces reactive oxygen species (ROS). Furthermore, the expression of AOX in cells and mice confirms that CI-CIII superassembly segments the CoQ in two pools and modulates CI-NADH oxidative capacity. |
