| Primary Identifier | MGI:4850027 | Allele Type | Targeted |
| Gene | Hoxd13 | Transmission | Germline |
| Strain of Origin | 129S2/SvPas | Is Recombinase | false |
| Is Wild Type | false |
| description | Cre recombinase-mediated recombination between the loxP site downstream of this insertion and a second loxP site elsewhere in the homologous chromosome may be used to delete the genes between the loxP sites, placing the gene following the deletion immediately downstream of Hoxd11 with its cis-acting regulatory elements. |
| molecularNote | The E. coli lacZ gene was inserted, with translational reading frames aligned, into the first exon of the mouse Hoxd11 gene in a construct containing the entire Hoxd11 transcription unit and its known cis-acting 5'and 3' regulatory sequences. A construct containing this fusion gene followed by an oppositely oriented loxP-flanked PGK-neo selection cassette was targeted to the intergenic region between the transcription initiation sites of Evx2 and Hoxd13, at the 5' extremity of the Hoxd complex, with Hoxd11/lacZ in the same transcriptional orientation as Hoxd13. The selection cassette was subsequently deleted by Cre recombinase-mediated recombination, leaving a single loxP site downstream/3' of the Hoxd/lacZ fusion gene. |
