| Primary Identifier | MGI:4867226 | Allele Type | Targeted |
| Attribute String | Conditional ready, Null/knockout, Reporter | Gene | Grn |
| Transmission | Germline | Strain of Origin | 129S1/SvImJ |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted upstream of exon 3. A cassette, which contains (5' to 3') a loxP site, FRT site, splice acceptor, IRES, beta-geo cassette, stop codon, polyadenylation sequence, and FRT site was inserted downstream of exon 4. The inserted sequence disrupts expression of endogenous Grn and instead expresses a lacZ. Immunohistochemistry and western blot analysis confirmed the lack of protein expression in the brain. |
