Primary Identifier | MGI:4867441 | Allele Type | Targeted |
Attribute String | Inducible, Recombinase | Gene | Sox9 |
Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<+> |
Induced With | tamoxifen | Is Recombinase | true |
Is Wild Type | false |
molecularNote | A targeting cassette containing IRES-cre/ERT2-pA and FRT-flanked PGK-neo-bpA sequences was introduced into AB1 ES cells. Homologous recombination inserted into the 3' UTR of exon 3 of the Sox9 locus. Targeted ES cells were injected into C57BL/6 blastocysts to produce chimeras which were crossed with C57BL/6 wild-type animals. The neo cassette was not removed from the targeted allele. |