| Primary Identifier | MGI:4880251 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Dhrs3 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | NorCOMM |
| description | Cell lines N01359P1_C_97W_B2 and N01359P1_C_97W_G1 were successfully used to make chimeric mice. Germline transmission was accomplished. J:214109. |
| molecularNote | The insertion of NorCOMM cassette pGOHANU created a deletion of size 6002bp between positions 144644767-144650769 of Chromosome 4 (Build GRCm39). This deletion results in the removal of functionally critical exons 2-5. The cassette is composed of an F3 site followed by a universal inverse PCR primer site, splice acceptor IRES-lacZ sequence and a loxP site. This first loxP site is followed by deltaTK and neomycin under the control of the human beta-actin promoter / deltaTK / neomycin phosphotransferase (neoR) gene / SV40 polyA and a second loxP site. After the second loxP site is a PhiC31 attP site and a puromycin sequence that is not active unless a promoter is inserted by PhiC31 at the attP site. The cassette is finished with an FRT site. Variations of this allele can be made using PhiC31 docking and subsequent cloning (Nagy et al., 2009. Methods Mol. Biol. 530:365-78). Western blot confirmed absence of protein in E14.5 embryonic fibroblasts. |
