Primary Identifier | MGI:4887403 | Allele Type | Targeted |
Gene | Gnas | Transmission | Germline |
Strain of Origin | 129S/SvEv | Is Recombinase | false |
Is Wild Type | false |
description | ES cell = MAC3. |
molecularNote | A rabbit beta-globin polyadenylation cassette was inserted into the end of the exon 1A in reverse orientation so that it does not truncate the exon 1A transcript. A loxP flanked neomycin cre cassette was inserted immediately downstream as well. RT-PCR analysis indicated reduced mRNA levels were produced. Northern blot analysis did not detect mRNA. |