Other
11 Authors
- Wei L,
- Ward SM,
- Jorgensen BG,
- Lee MY,
- Fuchs R,
- Ha SE,
- Redelman D,
- Park C,
- Park PJ,
- Sanders KM,
- Ro S
| First Author | Lee MY | Year | 2017 |
| Journal | PLoS One | Volume | 12 |
| Issue | 4 | Pages | e0176031 |
| PubMed ID | 28426719 | Mgi Jnum | J:248065 |
| Mgi Id | MGI:5917412 | Doi | 10.1371/journal.pone.0176031 |
| Citation | Lee MY, et al. (2017) Transcriptome of interstitial cells of Cajal reveals unique and selective gene signatures. PLoS One 12(4):e0176031 |
| abstractText | Transcriptome-scale data can reveal essential clues into understanding the underlying molecular mechanisms behind specific cellular functions and biological processes. Transcriptomics is a continually growing field of research utilized in biomarker discovery. The transcriptomic profile of interstitial cells of Cajal (ICC), which serve as slow-wave electrical pacemakers for gastrointestinal (GI) smooth muscle, has yet to be uncovered. Using copGFP-labeled ICC mice and flow cytometry, we isolated ICC populations from the murine small intestine and colon and obtained their transcriptomes. In analyzing the transcriptome, we identified a unique set of ICC-restricted markers including transcription factors, epigenetic enzymes/regulators, growth factors, receptors, protein kinases/phosphatases, and ion channels/transporters. This analysis provides new and unique insights into the cellular and biological functions of ICC in GI physiology. Additionally, we constructed an interactive ICC genome browser (http://med.unr.edu/physio/transcriptome) based on the UCSC genome database. To our knowledge, this is the first online resource that provides a comprehensive library of all known genetic transcripts expressed in primary ICC. Our genome browser offers a new perspective into the alternative expression of genes in ICC and provides a valuable reference for future functional studies. |
