| Primary Identifier | MGI:4940842 | Allele Type | Transgenic |
| Attribute String | Constitutively active, Humanized sequence, Inducible, Inserted expressed sequence | Gene | Tg(tetO-ABL1*P242E*P249E)CPdav |
| Strain of Origin | FVB/NTac | Induced With | doxycycline/tetracycline |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The TRE-AblPP transgene was designed with a Tet response element (TRE) followed by a constitutively active c-Abl sequence (AblPP) and a beta-globin polyA sequence. The Tet response element (TRE) contains seven copies of the 42-bp tet operator sequence (tetO) just upstream of a minimal CMV promoter. To generate the AblPP sequence, a cDNA sequence encoding the nuclear-localizing isoform (1b) of the human c-Abl (ABL1; c-abl oncogene 1, non-receptor tyrosine kinase) was modified via site-directed mutagenesis to change the conserved proline residues of the SH2-SH3 linker region to glutamate (P242E/P249E). |
