| Primary Identifier | MGI:4946645 | Allele Type | Targeted |
| Attribute String | Conditional ready, Humanized sequence, Hypomorph | Gene | Braf |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An FRT site and a loxP site flanked hygromycin resistance gene and STOP cassette were inserted in intron 17 (in ENSMUST00000002487), an ENSMUST00000002487:c.1910T>A mutation was created in exon 18 (changing valine codon 637 (GTG) to aspartic acid (GAG) (p.V637E)) and an FRT site was inserted into intron 18. RT-PCR analysis indicates the mutant allele is hypomorphic, expressed at about 5-10% of the level of the wild-type allele. The mutation is the equivalent of the human c.1799T>A (p.V600E) mutation, the most common oncogenic mutation in BRAF. |
