| Primary Identifier | MGI:4949465 | Allele Type | Transgenic |
| Attribute String | Inducible, Inserted expressed sequence, Reporter | Gene | Tg(tetO-TPR/MET,-EGFP)12Tcre |
| Strain of Origin | FVB | Induced With | doxycycline/tetracycline |
| Is Recombinase | false | Is Wild Type | false |
| description | Multiple founders whose fibroblasts expressed EGFP when infected with a tTA-expressing lentivirus under a ubiquitous promoter were used to establish mouse lines. Founder #12 has two independent insertions which segregate from one another. Sublines #12.2 and #12.9 were used in the reported studies. (J:120883) |
| molecularNote | A bi-directional tetracycline response element (TetO) drives expression of an enhanced green fluorescent protein reporter gene (EGFP) and of a cDNA encoding the human TRP-MET oncoprotein, in which the N-terminal of TPR (translocation promoter region), containing a pair of dimerization sites, is joined to the tyrosine kinase domain of the MET proto-oncogene, which is the receptor for hepatocyte growth factor. Crossing mice bearing this transgene with mice that express the tetracycline transactivator (tTA) or reverse transactivator (rtTA) protein under the control of a tissue-specific promoter permits regulation by tetracycline withdrawal/administration of the expression of TRP-MET and of the GFP reporter in the progeny. |
