| First Author | Prajapati M | Year | 2021 |
| Journal | J Biol Chem | Volume | 297 |
| Issue | 1 | Pages | 100835 |
| PubMed ID | 34051234 | Mgi Jnum | J:306702 |
| Mgi Id | MGI:6713201 | Doi | 10.1016/j.jbc.2021.100835 |
| Citation | Prajapati M, et al. (2021) Biliary excretion of excess iron in mice requires hepatocyte iron import by Slc39a14. J Biol Chem :100835 |
| abstractText | Iron is essential for erythropoiesis and other biological processes, but is toxic in excess. Dietary absorption of iron is a highly regulated process and is a major determinant of body iron levels. Iron excretion, however, is considered a passive, unregulated process, and the underlying pathways are unknown. Here we investigated the role of metal transporters SLC39A14 and SLC30A10 in biliary iron excretion. While SLC39A14 imports manganese into the liver and other organs under physiological conditions, it imports iron under conditions of iron excess. SLC30A10 exports manganese from hepatocytes into the bile. We hypothesized that biliary excretion of excess iron would be impaired by SLC39A14 and SLC30A10 deficiency. We therefore analyzed biliary iron excretion in Slc39a14- and Slc30a10-deficient mice raised on iron-sufficient and -rich diets. Bile was collected surgically from mice then analyzed with non-heme iron assays, mass spectrometry, ELISAs, and an electrophoretic assay for iron-loaded ferritin. Our results support a model in which biliary excretion of excess iron requires iron import into hepatocytes by SLC39A14, followed by iron export into bile predominantly as ferritin, with iron export occurring independently of SLC30A10. To our knowledge, this is the first report of a molecular determinant of mammalian iron excretion, and can serve as basis for future investigations into mechanisms of iron excretion and relevance to iron homeostasis. |
